From Cellprofiler Developer Wiki

We are pleased to announce the release of CellProfiler 2.0.

Contents 1 CellProfiler 2.0 Release Notes 1.1 Features 1.2 Limitations 1.3 Unimplemented Modules and Data Tools 1.4 Known Bugs (4th Beta r10211) 1.4.1 IMG-543 If Excel has LoadData’s text file open, LoadData’s setting check fails

CellProfiler 2.0 Release Notes

Features

For the second release (10415)

• LoadImages can now load images by order.
• CorrectIlluminationCalculate has a new spline method for determining the background intensity across an image.
• We've added a new module, UnmixColors, which performs color deconvolution for histologically-stained images.
• MeasureGranularity can now measure the granularity of objects.
• We have a metadata editor for settings that can use metadata substitution, for instance, the file names in SaveImages.
• CellProfiler uses Bioformats version 4.2 to load and save many file formats; this is a stable, heavily-tested version.
• CellProfiler can run ImageJ macros on the Macintosh
• Plugins support: Write your own module for CellProfiler
• Many bug fixes (see Fixes_by_version).

For first non-Beta (10211) and 3rd Beta (9978)

• Many bug fixes (see Fixes_by_version).

For 2nd Beta (9777)

• Build for 64-bit Windows system now available.
• Module display windows use less memory.
• Fix bug in LoadImages when trying to load flex files.
• Fix crash on Snow Leopard when "Analyze Images" button is pushed.
• Bundle JRE on windows to avoid install problems.

For Beta (9722, 9764)

• Orientation screen on startup
• Bug fixes
• Complete rewrite in python.
• Many new features: See here

Limitations

The 32-bit Windows release may run out of memory when run on large images, pipelines that use many images simultaneously or pipelines that open many module display windows. We've taken steps to plug memory leaks and reduce the amount of memory that module display windows take up.

However, if memory issues still occur, Windows users should use the ConserveMemory module to delete intermediate images, should only keep the windows that they need open and should consider resizing very large images before processing with their pipeline.

Alternately, users with 64-bit machines may use our new 64-bit beta Windows release (r9777) which will help with some of the memory issues.

Unimplemented Modules and Data Tools

Details on changed or missing modules/functionality can be found here

Known Bugs (4th Beta r10211)

IMG-543 If Excel has LoadData’s text file open, LoadData’s setting check fails

CellProfiler might not operate correctly if a .csv file used by LoadData is being used by Excel or some other program simultaneously due to file sharing issues.