Module: RelateObjects

Relate Objects assigns relationships; all objects (e.g. speckles) within a parent object (e.g. nucleus) become its children.
This module allows you to associate child objects with parent objects. This is useful for counting the number of children associated with each parent, and for calculating mean measurement values for all children that are associated with each parent.

An object will be considered a child even if the edge is the only part touching a parent object. If an child object is touching multiple parent objects, the object will be assigned as a child of all parents that it overlaps with.

Available measurements

Parent object measurements:

Child object measurements:

See also: ReassignObjectNumbers.

Settings:

Select the input child objects

Child objects are defined as those objects contained within the parent object. For example, when relating speckles to the nuclei that contains them, the speckles are the children.

Select the input parent objects

Parent objects are defined as those objects which encompass the child object. For example, when relating speckles to the nuclei that contains them, the nuclei are the parents.

Calculate child-parent distances?

Choose the method to calculate distances of each child to its parent.

Calculate per-parent means for all child measurements?

Select Yes to calculate the per-parent mean values of every upstream measurement made with the children objects and stores them as a measurement for the parent; the nomenclature of this new measurements is "Mean_<child>_<category>_<feature>". For this reason, this module should be placed after all Measure modules that make measurements of the children objects.

Calculate distances to other parents?

(Used only if calculating distances)
Select Yes to calculate the distances of the child objects to some other objects. These objects must be either parents or children of your parent object in order for this module to determine the distances. For instance, you might find "Nuclei" using IdentifyPrimaryObjects, find "Cells" using IdentifySecondaryObjects and find "Cytoplasm" using IdentifyTertiaryObjects. You can use Relate to relate speckles to cells and then measure distances to nuclei and cytoplasm. You could not use RelateObjects to relate speckles to cytoplasm and then measure distances to nuclei, because nuclei is neither a direct parent or child of cytoplasm.

Parent name

(Used only if calculating distances to another parent)
Choose the name of the other parent. The RelateObjects module will measure the distance from this parent to the child objects in the same manner as it does to the primary parents. You can only choose the parents or children of the parent object.