places outlines produced by an Identify
module over a desired image.
This module places outlines (in a special format produced by an Identify
module) on any desired image (grayscale, color, or blank). The resulting image can be saved using the SaveImages
See also IdentifyPrimaryObjects, IdentifySecondaryObjects, IdentifyTertiaryObjects.
Display outlines on a blank image?
to produce an
image of the outlines on a black background.
Select No, the module will overlay the
outlines on an image of your choosing.
Select image on which to display outlines
(Used only when a blank image has not been selected)
Choose the image to serve as the background for the outlines.
You can choose from images that were loaded or created by modules
previous to this one.
Name the output image
Enter the name of the output image with the outlines overlaid.
This image can be selected in later modules (for instance, SaveImages).
Outline display mode
Specify how to display the outline contours around
your objects. Color outlines produce a clearer display for
images where the cell borders have a high intensity, but take
up more space in memory. Grayscale outlines are displayed with
either the highest possible intensity or the same intensity
as the brightest pixel in the image.
Select method to determine brightness of outlines
(Used only when outline display mode is grayscale)
The following options are possible for setting the intensity
(brightness) of the outlines:
- Max of image: Set the brighness to the
the same as the brightest point in the image.
- Max possible: Set to the maximum
possible value for this image format.
If your image is quite dim, then putting bright white lines
onto it may not be useful. It may be preferable to make the
outlines equal to the maximal brightness already occurring
in the image.
Width of outlines
Enter the width, in pixels, of the
outlines to be displayed on the image.
Select outlines to display
Choose outlines to display, from a previous Identify
module. Each of the Identify modules has a checkbox that
determines whether the outlines are saved. If you have checked this,
you were asked to supply a name for the outline; you
can then select that name here.
Load outlines from an image or objects?
Prior versions of OverlayOutlines would only
display outline images which were optional outputs of the identify
modules. For legacy pipelines or to continue using the outline
images instead of objects, choose Image. Choose
Objects to create the image directly from the
objects. This option will improve the functionality of the
contrast options for this module's interactive display and will
Select objects to display
Choose the objects whose outlines you would like