Module: MeasureObjectRadialDistribution

Measure Object Radial Distribution measures the radial distribution of intensities within each object.
Given an image with objects identified, this module measures the intensity distribution from each object's center to its boundary within a user-controlled number of bins, i.e. rings.

The distribution is measured from the center of the object, where the center is defined as the point farthest from any edge. The numbering is from 1 (innermost) to N (outermost), where N is the number of bins specified by the user. Alternatively, if primary objects exist within the object of interest (e.g. nuclei within cells), you can choose the center of the primary objects as the center from which to measure the radial distribution. This might be useful in cytoplasm-to-nucleus translocation experiments, for example. Note that the ring widths are normalized per-object, i.e., not necessarily a constant width across objects.

Available measurements

See also MeasureObjectIntensity.

Settings:

Select an image to measure

Select the image that you want to measure the intensity from.

Select objects to measure

Select the objects that you want to measure the intensity from.

Object to use as center?

There are three ways to specify the center of the radial measurement: For example, if measuring the radial distribution in a Cell object, you can use the center of the Cell objects (These objects) or you can use previously identified Nuclei objects as the centers (Centers of other objects).

Select objects to use as centers

(Used only if "Centers of other objects" are selected for centers)
Select the object to use as the center, or select None to use the input object centers (which is the same as selecting These objects for the object centers).

Scale the bins?

Select Yes to divide the object radially into the number of bins that you specify.

Select No to create the number of bins you specify based on distance. For this option, the user will be asked to specify a maximum distance so that each object will have the same measurements (which might be zero for small objects) and so that the measurements can be taken without knowing the maximum object radius before the run starts.

Number of bins

Specify the number of bins that you want to use to measure the distribution. Radial distribution is measured with respect to a series of concentric rings starting from the object center (or more generally, between contours at a normalized distance from the object center). This number specifies the number of rings into which the distribution is to be divided. Additional ring counts can be specified by clicking the Add another set of bins button.

Maximum radius

Specify the maximum radius for the unscaled bins. The unscaled binning method creates the number of bins that you specify and creates equally spaced bin boundaries up to the maximum radius. Parts of the object that are beyond this radius will be counted in an overflow bin. The radius is measured in pixels.