Module: MakeProjection

Make Projection combines several two-dimensional images of the same field of view together, either by performing a mathematical operation upon the pixel values at each pixel position.
This module combines a set of images by performing a mathematic operation of your choice at each pixel position; please refer to the settings help for more information on the available operations. The process of averaging or summing a Z-stack (3D image stack) is known as making a projection.

This module will create a projection of all images specified in the Input modules. For more information on loading image stacks and movies, see Help > Creating a Project > Loading Image Stacks and Movies. To achieve per-folder projections i.e., creating a projection for each set of images in a folder, for all input folders, make the following setting specifications:

  1. In the Images module, drag-and-drop the parent folder containing the sub-folders.
  2. In the Metadata module, enable metadata extraction and extract metadata from the folder name by using a regular expression to capture the subfolder name, e.g., .*[\\/](?P<Subfolder>.*)$
  3. In the NamesAndTypes module, specify the appropriate names for any desired channels.
  4. In the Groups module, enable image grouping, and select the metadata tag representing the sub-folder name as the metadata category.
Keep in mind that the projection image is not immediately available in subsequent modules because the output of this module is not complete until all image processing cycles have completed. Therefore, the projection should be created with a dedicated pipeline.

See also the help for the Input modules.


Select the input image

Select the image to be made into a projection.

Type of projection

The final projection image can be created by the following methods:


Name the output image

Enter the name for the projected image.


(Used only if Power is selected as the projection method)
This setting controls the frequency at which the power is measured. A frequency of 2 will respond most strongly to pixels that alternate between dark and light in successive z-stack slices. A frequency of N will respond most strongly to pixels whose brightness cycle every N slices.