Module: EnhanceOrSuppressFeatures

Enhance Or Suppress Features enhances or suppresses certain image features (such as speckles, ring shapes, and neurites), which can improve subsequent identification of objects.
This module enhances or suppresses the intensity of certain pixels relative to the rest of the image, by applying image processing filters to the image. It produces a grayscale image in which objects can be identified using an Identify module.

Settings:

Select the input image

Select the image with features to be enhanced or suppressed.

Name the output image

Enter a name for the feature-enhanced or suppressed image.

Select the operation

Select whether you want to enhance or suppress the features you designated.

Feature size

(Used only if circles, speckles or neurites are selected, or if suppressing features)
Enter the diameter of the largest speckle, the width of the circle or the width of the neurites to be enhanced or suppressed, which will be used to calculate an adequate filter size. To view pixel intensities in an open image, use the pixel intensity tool which is available in any open display window. When you move your mouse over the image, the pixel intensities will appear in the bottom bar of the display window.

Feature type

(Used only if Enhance is selected)
This module can enhance three kinds of image intensity features: In addition, this module enables you to suppress certain features (such as speckles) by specifying the feature size.

Range of hole sizes

(Used only if Dark holes is selected)
The range of hole sizes to be enhanced. The algorithm will identify only holes whose diameters fall between these two values.

Smoothing scale

(Used only for the Texture, DIC or Neurites methods)
  Smoothing can be turned off by entering a value of zero, but this is not recommended.

Shear angle

(Used only for the DIC method)
The shear angle is the direction of constant value for the shadows and highlights in a DIC image. The gradients in a DIC image run in the direction perpendicular to the shear angle. For example, if the shadows run diagonally from lower left to upper right and the highlights appear above the shadows, the shear angle is 45°. If the shadows appear on top, the shear angle is 180° + 45° = 225°.

Decay

(Used only for the DIC method)
The decay setting applies an exponential decay during the process of integration by multiplying the accumulated sum by the decay at each step. This lets the integration recover from accumulated error during the course of the integration, but it also results in diminished intensities in the middle of large objects. Set the decay to a large value, on the order of 1 - 1/diameter of your objects if the intensities decrease toward the middle. Set the decay to a small value if there appears to be a bias in the integration direction.

Enhancement method

(Used only for the Neurites method)
Two methods can be used to enhance neurites: