Correct Illumination - Apply
applies an illumination function, usually created by CorrectIlluminationCalculate
, to an image in order to correct for uneven illumination (uneven shading).
This module applies a previously created illumination correction function, either loaded by LoadSingleImage or created by CorrectIlluminationCalculate. This module corrects each image in the pipeline using the function specified.
See also CorrectIlluminationCalculate.
Select the input image
Select the image to be corrected.
Name the output image
Enter a name for the corrected image.
Select the illumination function
Select the illumination correction function image that will be used to
carry out the correction. This image is usually produced by another module
or loaded as a .mat format image using the Images module or
Select how the illumination function is applied
This choice depends on how the illumination function was calculated
and on your physical model of the way illumination variation affects the
background of images relative to the objects in images; it is also somewhat empirical.
- Subtract: Use this option if the background signal is significant
relative to the real signal coming from the cells. If you created the illumination
correction function using Background,
then you will want to choose Subtract here.
- Divide: Choose this option if the the signal to background ratio
is high (the cells are stained very strongly). If you created the illumination correction
function using Regular,
then you will want to choose Divide here.