How to identify nuclei in my DIC picture

Ask all of your CellProfiler 1.0 questions here.

Moderators: mbray, LeeKamentsky, Anne Carpenter, davidlogan

How to identify nuclei in my DIC picture

Postby lily0204 » Tue Mar 23, 2010 2:04 am

Hi, Cellprofiler is quite new for me? Could anyone help me to figure out how to identify the nuclei in my DIC picture?
Thanks.
Attachments
HeLA.wtK.con.3h.ph.01.tif
HeLA.wtK.con.3h.ph.01.tif (1.28 MiB) Viewed 2399 times
lily0204
 
Posts: 1
Joined: Sun Mar 21, 2010 3:15 pm

Re: How to identify nuclei in my DIC picture

Postby mbray » Mon Mar 29, 2010 11:55 am

CellProfiler is optimized for fluorescent images. Unless your DIC images have good contrast, cell segmentation is unlikely to work well, much less sub-compartment segmentation (such as identifying nuclei from the cell). In this case, the DIC image seems to be saturated and the outlines of the nuclei, though somewhat visible by eye, will probably not be distinguishable by automated means.

Still, take a look at forum page under item 5. You may find some helpful hints there.

Regards,
-Mark
Mark Bray
Computational Biologist/Image Assay Developer
Carpenter Lab, The Broad Institute
User avatar
mbray
 
Posts: 2128
Joined: Tue Apr 29, 2008 8:40 am
Location: Broad Institute, Cambridge, MA


Return to CellProfiler 1.0 Help

Who is online

Users browsing this forum: No registered users and 1 guest

cron